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    盐胁迫对白榆无性系抗氧化酶活性及丙二醛的影响

    Effects of salt stress on activities of antioxidant enzymes and MDA of elm clones

    • 摘要: 为了促进滨海盐渍荒漠化地区的植被恢复、引种栽培与盐碱土的改良利用,以3 个白榆无性系Y65225、Y46和Y51 为试验材料,采用盆栽模拟方法,分析白榆无性系在不同浓度(0.0%、0.3%、0.5%、0.7% 和0.9%)NaCl 处理下抗氧化酶(SOD、POD 和CAT)活性、丙二醛(MDA)含量变化及各指标的相关性,并运用隶属函数法,综合评价3 个白榆无性系的耐盐性。结果表明:在各盐浓度胁迫下,Y51 能够维持较高的抗氧化酶活性,抗氧化能力最强;Y46 的抗氧化酶活性下降最大,抗氧化能力最差,Y46 的MDA 含量增加幅度最大,受到的盐害最大;Y51的MDA 含量增加幅度最小,受到的盐害最小。3 个白榆无性系的SOD 活性与POD 活性,呈极显著正相关,CAT 活性与SOD活性、POD 活性相关性不显著,CAT、POD 活性与MDA 含量相关性不强,SOD 活性与MDA 含量呈显著负相关关系,即SOD 活性的增加,可显著减少MDA 的积累,增强其抗盐性。综合各项指标,Y51 抗盐能力最强,Y65225 次之,Y46 最差。建议在滨海盐碱地区造林时,优先采用Y51 无性系。研究结果将为今后白榆无性系在盐碱地的栽培,提供一定的参考。

       

      Abstract: Background The coastal saline soil is formed by the seawater immersion in the river sediments, with salting and salt characteristics of a typical modern seawater effects. The typical characteristic of coastal saline-alkali soil is that the content of NaCl and the groundwater level are high.Chlorides, sulfates and salt content in the soil directly affect the survival of the germination of seeds, limiting the growth and yield of plants, which has become the most direct factor of vegetation restoration in coastal saline-alkali soil area. Ulmus pumila L. is the most widely distributed and important fast growing hardwood timber species that has solid tolerance to drought, cold, salt and wind. It is the most important timber species in vast north plains, salinealkali areas and desert to construct useful afforestation, shelter forest and greenings. Methods In order to cultivate, modify and utilize salinized soil of coastal saline area, 3 elm clones (Y65225, Y46 and Y51) were chosen as test materials, watering with 5 salt concentrations of 0.0%, 0.3%, 0.5%, 0.7%, and 0.9% by pot-cultivated experiments in the greenhouse. The activities of dismutase (SOD), catalase (CAT), peroxidase (POD) and mass content of malondialdehyde (MDA) were determined in this study. The change of antioxidant enzymes activity and mass content of MDA and the correlation of the indexes were analyzed. In addition, salt resistance of the 3 clones was comprehensively evaluated by subordination function method. Results Y51 sustained high antioxidant enzyme activity under different salt concentrations, meaning it had the strongest antioxidant capacity. While as the antioxidant capacity of Y46 was the worst, because its antioxidant enzyme activity decreased in greatest. MDA was an important indicator of injured cell membrane, which shows that the seedlings are efficiently influenced by the membrane lipid peroxidation. The activities of MDA increased with the salt stress increasing, the greatest increase was from Y46, while the smallest from Y51 and the salt injury to Y51 was the least. The correlation analysis of the measurement indicators for 3 elm clones showed that the correlation of SOD activity and POD activity were significant; besides the CAT activity presented no significant correlation with the above two indicators. The activities of CAT and POD had little significance with the mass content of MDA. Moreover, the activity of SOD showed certain negative correlation with the mass content of MDA, i. e., the increase of SOD significantly reduced the accumulation of MDA mass content, and therefore enhanced the salt resistance of the elm clones. Conclusions Ultimately, through overall assessment, salt resistance of Y51 was the highest, less for Y65225 and the lowest for Y46, indicating that they belonged to the middling salt-tolerant elm clones. We recommend that Y51 is the preferred clone in afforestation in the coastal areas. The results of this paper may also provide a certain theoretical significance for cultivating elm clones in the future.

       

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