Abstract: Agricultural non-point source pollution has become the major factor of water quality deterioration. By identifying agricultural non-point source pollution control zones according to the environmental quality standard requirements of different water function zones of the nation and provinces, the management efficiency for controlling non-point source pollution can be improved. Taking 103 counties of Hubei Province as basic units, based on the demand of water function zones, this paper identified the environmental quality standards for surface water at county level. On this basis, the agricultural non-point source pollution type and environmental sensitivity of each county were determined by using the method of water quality pollution index and Nemoro index. Finally, the pollution control zones were determined. The results show that, 1) 29 counties are divided into Class-Ⅱ water quality protection area and 74 counties into Class-Ⅲ water quality protection area; 2) in Class-Ⅱ area, the pollution type is divided into three types, which are all single source pollution, and the environmental sensitive area was divided into three regions, namely, non-sensitive, slightly sensitive and moderately sensitive regions; 3) in Class-Ⅲ area, there are seven pollution types and main pollution types are single source pollution and mixed pollution, and the environmentally sensitive area includes three regions, namely, slightly, moderately and seriously sensitive regions; 4) the pollution control zone is divided into three classes: the first class includes two regions, the second class includes six regions and the third class includes 23 regions. Our results can contribute to providing a basis for pollution management and prevention in Hubei Province.