Abstract: Guizhou is a typical Karst area in the Southwest of China,there are rich of biological and mineral resources,water and tourism resources.However,due to prominent human-land contradiction,tender ecological environment,disadvantageous weather and laggard production habits,the sustainable development was seriously restricted.Author analyzed thoroughly the resources superiority and the restrict factors of the Karst area in Guizhou Province,then,proposed the increasing of the eco-environment construction,adjusting of industrial structure,speeding technical innovation step,and so on,for the sustainable development of Karst area in Guizhou Province.